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Overview

Features:

The ProtOn™ Biotin Labeling Kit is designed for simple and fast labeling of proteins with biotin.

The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules that have primary amino groups.

Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction).

Kit components:

Labeling Reagent (Biotin [Long Arm] NHS)

Reagent Solvent

Reaction Buffer

Stop Reagent

Gel Filtration Slurry

5 Spin Columns

5 Collection Tubes

Agarose Avidin D Slurry

Cat. No. PLK-1202

Unit Size 1 kit

Country of Manufacture United States

Target for Labeling Protein

Required Reactive Group Primary Amino Group

Tag/Group Incorporated Biotin

More Information

Protein labeling and modification is a powerful tool for the isolation and analysis of proteins. The ProtOn™ Protein Labeling Kits simplify the attachment of either biotin or fluorescein to antibodies or other proteins. Vector Laboratories offers a variety of reagents and kits for protein labeling allowing for greater flexibility of experimental design.

This ProtOn™ protein labeling kit is designed for the simple and fast labeling of proteins with biotin. The kit is ideally suited to label antibodies, hormones, or other proteins as well as other macromolecules containing primary amino groups. Labeled proteins can be used in a number of applications, such as immunological assays, fluorescence microscopy, investigation of protein-protein interactions, etc.

Kit Contents:

Each kit contains all the components needed for at least five labeling reactions (up to 1 mg of protein per reaction). Kit components include:

Labeling Reagent (Biotin [Long Arm] NHS)

Reagent Solvent

Reaction Buffer

Stop Reagent

Gel Filtration Slurry

5 x 1 ml Spin Columns

5 x 2 ml Collection Tubes

Agarose Avidin D Slurry

The ProtOn™ labeling reagents react with primary amines (i.e. lysine residues or terminal amino groups) on the protein, forming a covalent bond. The linkage between the label and the protein produced with these reagents is stable, with no significant loss of the label during storage.

Each ProtOn™ Kit is supplied with complete instructions that offer the researcher a step-by-step guide to convenient and easy protein labeling with biotin or fluorescein.

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