抗荧光衰退封片剂,价格,厂家,上海桑戈供
抗荧光衰退封片剂
1.抗荧光衰退封片剂(CITIFLUOR™ - Antifadent Mountant Solutions)
适于荧光封片。由于生物样品的多样性以及免疫荧光组化中荧光衰退的复杂性,并没有一个完全通用的抗荧光衰退剂,常见的抗荧光衰退剂有AF1、AF2、AF3、AF87,其中AF87是一个一个的油性抗荧光衰退剂。
保存温度 货号 品名 规格
RT 17970-25 CitiFluor™ AF1 25 ml
RT 17971-25 CitiFluor™ AF2 25 ml
RT 17972-25 CitiFluor™ AF3 25 ml
RT 17976-10 CitiFluor™ AF87 10 ml
RT 17978-35 Set of CitiFluor™ CFPVOH and AF100* 25 ml+ 5 ml
产品选购指南:
There is no universal antifadent due to the fact that biological material is so varied in nature that the fluorescence fading of fluorochromic conjugates is a complex process.
The general anti-fadents are AF1, AF2, and AF3. The AF87 is a specialist immersion oil-based antifadent.
AF1 (Glycerol-phosphate buffered solution containing an additive for use with labeled tissue sections). Should be used if you normally examine specimens in glycol solution. If you normally use saline solution with your specimens.
AF2 (Glycerol solution containing additives for use with labeled tissue sections) is simply a glycerol solution of the antifadent.
AF3 (Phosphate-buffered saline solution containing additives for examination of whole cell) is appropriate.
AF87, the antifadent, is contained in non-fluorescent immersion oil. It is designed for those requiring work at high magnification. It is useful for preventing the fading of such dyes as DAPI, which have the tendency to disperse in glycerol based mountant solutions.
CFPVOH is an aqueous solution of poly(vinyl alcohol) for use as a solid CFPVOH is an aqueous solution of poly (vinyl alcohol) for use as a solid mountant. Apply a few drops of the solution to your sample, and allow drying to give a clear film.
AF100 is a solution of antifadents to be used with CFPVOH when fading of fluorescence in the poly(vinyl alcohol) is a problem. To prepare a CFPVOH solution containing AF100, add 0.1ml of Af100 to 0.9ml of CFPVOH. If more or less of the solid mountant solution is required, mix the reagent in the appropriate ratio. Once these solutions have been made up, they should be used within a period of days since the solution deteriorates on storage.
All of these products have a long shelf life, a minimum of 2 years. The solution can be stored at room temp-erature. With LD50’s >2000mg/kg (in rat), this product exhibits no toxicological problems.
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